The performance and application of  TMB:
3,3',5,5'-Tetramethylbenzidine(TMB) is a new-type safe color primary reagent.Compared with some traditional color primary
reagents such as benzidine, o-tolidine and o-diaminobenzene,the merits of TMB are high sensitive detection and excellent
stability. And as a result of zoopery and Ames which is to experiment on self-acting variability of bacteria,showing that TMB is
a kind of non-cancerogen and non-mutagen ,and can be used safely. Currently,TMB is taking the place of benzidine which is
a strong cancerogen and other ramifications of benzidine which are  carcinogenic step by step.TMB can be applied in areas
like clinical assay, forensic analysis, criminal investigation and environmental detection,etc.As a new zymolyte of peroxidase,
TMB has wide applications in enzyme immunoassay (EIA) and enzyme-linked-immunosorbent serologic assay (ELISA).
 
The main items of TMB applications are:
1.making up test paper of uranalysis;
2.detection of hepatitis virus;
3.detection of gravidity;
4.rapid determination of glucose,hemoglobin and albumin in blood and urine;
5.occult blood test of excrement;
6.to detect the value of granulocyte in blood;
7.detection of steroid and sex hormone;
8.determination of enzyme activity;
9.analysis and detection of antigen, antibody and germ plasm;
10.rapid determination of alcoholicity in saliva;
11.pigmentation of biological specimen;
12.quality test of water and detection of fingerprint rendering;
13.detection of AIDS virus.
14.ELISA Kit substrates.